The National Institutes of Health is under fire for spending taxpayer money on experiments that pumped male macaque monkeys with estrogen, feminizing them before testing their immune response to mRNA vaccines.
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The study, appearing in Cell Reports, detailed how researchers induced female traits in male monkeys by administering feminizing hormone therapy (FHT) drug pellets that suppressed testosterone production, followed by an mRNA vaccine. The results were then compared against monkeys that received placebos.
Researchers noted the monkeys that received FHT developed the expected physical sex changes.
FHT induces physical changes in TGW (transgender women), such as breast development, fat and muscle redistribution, and reduction in facial hair. To determine whether exogenous E2 (17β-estradiol) therapy triggered similar female characteristics in male [rhesus macaques], we evaluated body alterations in the E2-treated animals. We found that male [rhesus macaques]treated with E2, but not placebo, developed significantly enlarged nipples similar to those of non-pregnant non-lactating female macaques.
“Interestingly,” researchers continued, “the skin in the E2-treated macaques’ hips and thighs also became increasingly reddish and vascularized in a manifestation that resembled sex skin, which is triggered by sex hormones and serves as a visual display of reproductive status in NHPs (nonhuman primates).”
Researchers ultimately discovered that hormone-treated monkeys had a stronger immune response and higher T cell activity after receiving an mRNA vaccine — however, the activated cells are the same ones targeted by the human immunodeficiency virus (HIV), suggesting HRT could increase susceptibility to HIV infection.
Along these lines, TGW (transgender women/biological men) living without HIV-1 and taking FHT harbor significantly higher frequencies of CCR5+ CD4+ T cells in gut mucosa than cisgender women and men who have sex with men. Furthermore, TGW living with HIV-1 and taking FHT have significantly higher CD4+ T cell counts, CD4:CD8 ratios, and total lymphocyte counts than cisgender women living with the virus. This target cell expansion could contribute to the disproportionate burden of HIV-1 infection shouldered by TGW, who are 49–66 times more likely to be living with HIV-1 than cisgender adults.
The disturbing research was funded by the NIH under former Director Anthony Fauci, totaling $22 million.
The grants include U19AI149646 ($13.9 million to Boston Children’s Hospital for an AAV-mediated functional cure), R01HD103494 ($4 million to the University of Florida for preventing obstetric HIV infection), R01HD102252 ($4.1 million to UF for perinatal HIV prevention), and the expired R21AI157929 ($477,000 to UF specifically for studying immunological effects of FHT in transgender women models).
Additional support was provided by the State of Florida through the HIV/AIDS and Emerging Infectious Diseases Institute (HEIDI) at the University of Miami, which has been sponsored by the Florida Department of Health under contracts totaling millions of dollars since 2014.
In May, a group of Republican legislators, including Reps. Marjorie Taylor Greene (Ga.), Paul Gosar (Ariz.), Nancy Mace (S.C.) and Lauren Boebert (Colo.), wrote to the House Labor-HHS-Education Appropriations Subcommittee asking them to follow through on President Trump’s and the NIH’s initiative to cancel research grants on transgender animal testing and amend the “FY26 Labor, Health and Human Services, Education, and Related Agencies bill to ensure no more taxpayer dollars are wasted to fund transgender animal tests.”
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